A biosensor developed at Purdue University detects the movement of auxin along a plant’s root surface in real time without damaging the plant.
Marshall Porterfield, an associate professor of agricultural and biological engineering and biomedical engineering, created the sensor, which utilises black platinum and carbon nanotubes developed at Purdue.
The nanomaterials at the sensor’s tip react with auxin and create an electrical signal that can be measured to determine the auxin concentration at a single point.
The sensor is said to oscillate, taking concentration readings at different points around a plant root. An algorithm then determines whether auxin is being released or taken in by surrounding cells.
‘It is the equilibrium and transport dynamics that are important with auxin,’ said Porterfield, whose findings were published in the online version of The Plant Journal.
A current focus of auxin research is understanding how this hormone regulates root growth in plants growing on sub-optimal soils.
Angus Murphy, a Purdue professor of horticulture and the paper’s co-author, said that worldwide pressure on land for food and energy crops drives efforts to better understand how plant roots adapt to marginal soils. Auxin is one of the major hormones involved in that adaptive growth.
‘It’s the key effector of these processes,’ said Murphy.
Although sensors using similar nanomaterials have been in use for the real-time measurement of auxin levels along a root surface for several years, those earlier sensors required the application of external auxin at toxic levels as part of the measurement process.
Porterfield and Eric McLamore, a former Purdue post-doctoral researcher, created an algorithm to decode the information obtained from the sensor.
The algorithm processes the sensor information to show whether the hormone is moving into or out of cells. This allows the sensor to be self referencing, eliminates the need for auxin application and allows instantaneous and continuous measurements to be made during root growth.
Other current methods based on radioisotope tracers and auxin-responsive fluorescent proteins inserted into the plant can detect changes taking place over hours. Most auxin responses take place on a timescale of minutes.
Murphy said that auxin movement is key to how plants adapt to their environments. He said that the effort to develop the sensor with Porterfield originated with the need to improve real-time measurement capability and develop a method that allows comparison with other measurements to better understand how auxin transport and other biological functions are connected.
‘Using sensors such as this, we can get answers that just aren’t possible with existing tools,’ said Murphy. ‘Being able to measure the efflux and uptake simultaneously is really essential to a lot of ongoing work.’
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