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Lab M has introduced its Modified Giolitti and Cantoni Broth (ISO) for the detection and enumeration of coagulase-positive staphylococci, such as Staphylococcus aureus, from food and animal feed.

This versatile medium can be used with or without an agar/paraffin plug on the surface, depending upon the testing regime employed.

Modified Giolitti and Cantoni Broth is optimised for samples where staphylococci may be stressed and/or present only in low numbers.

Their growth is promoted by the inclusion of sodium pyruvate, glycine and high concentration mannitol.

Potassium tellurite, which inhibits other gram-positive organisms, and lithium chloride, which is inhibitory to Gram-negative bacilli, provide the necessary selectivity.

Tellurite also serves as an indicator for coagulase-positive staphylococci, its reduction by these organisms resulting in a blackening of the broth or the presence of a black precipitate.

The introduction of this Modified Giolitti and Cantoni Broth (ISO) enhances Lab M’s range of media for the isolation of coagulase-positive staphylococci in accordance with ISO 6888-3:2003.

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