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Lysozyme, also known as muramidase, is an enzyme found in human tears, saliva, human milk, and most white blood cells. Its function is to kill bacteria by damaging their cell walls through catalysing hydrolysis. Discovered in 1922 by Alexander Fleming, lysozyme became known as the first antibiotic.

Static light scattering (SLS) is an optical technique that measures the time-averaged intensity of scattered light. It is a typical method for determining molecular mass, especially of macromolecules such as polymers and proteins. Moreover, SLS can also be used to determine the second virial coefficient, which reflects the interaction between particles and solvent: A negative gradient indicates that the particle–solvent interactions are stronger than the particle–particle interactions, and vice versa.

Determination of molecular mass by SLS requires measurements at three or more different concentrations. Furthermore, a sample of the solvent, and of the reference must be freshly prepared and measured. The solvent sample must include all solutes (such as buffer or salt) that are contained in the sample other than the particles to be measured.

In the Application Report here available for download, the molecular mass of lysozyme is determined by using SLS measurements with the Litesizer 500, the particle analyser form Anton Paar for characterising nano- and microparticles in dispersions and solutions.

Follow the link above to have a full copy of the application report with the test results.

Anton Paar Ltd

Anton Paar develops, produces and distributes highly accurate laboratory instruments and process measuring systems, and provides custom-tailored automation and robotic solutions.

Anton Paar develops, produces and distributes highly accurate laboratory instruments and process measuring systems, and provides custom-tailored automation and robotic solutions.

It is the world leader in the measurement of density, concentration and CO2 and in the field of Rheometry. Other areas of specialty are: Viscometry, Polarimetry, Refractometry, Microwave Sample Preparation & Synthesis, Chemical and Mechanical Surface and Nanostructure Analysis.

With over 2200 employees at the headquarters in Graz (Austria) and the 25 sales subsidiaries worldwide, Anton Paar ensure that their products live up to their excellent reputation. The core competence of the company – high-precision production – and close contact to the scientific community form the basis for the quality of Anton Paar’s instruments.

A strong sales network in more than 110 countries guarantees customers rapid support and answers to their application and service queries.

Innovation is the basis for progress and every year Anton Paar invests more than 20 percent of the annual turnover in R&D.

Anton Paar Limited, a subsidiary of Anton Paar GmbH, provides sales, application support and service to users across the UK and Ireland.

With an office in St Albans (UK) and Dublin (Ireland) and a team of over 30 dedicated employees, Anton Paar Limited aims to provide first-class service at every stage in the customer relationship, ensuring our products live up to their excellent reputation.

Anton Paar Ltd
Unit F, The Courtyard
St Albans
AL4 0LA
UK

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