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Size-Determination-of-Vitamin-B1-by-Dynamic-Light-Scattering - .PDF file.

dynamic light scattering

Dynamic light scattering (DLS) is a physics technique used for characterising the size of particles and molecules in suspension and solution. The technique is based on the measurement of the time-dependent fluctuations in the intensity of the scattered light signals. The particles are responsible for the scattering of the light, while it is the random motion of the particles that produces the fluctuations in that scattering. By measuring the scattering, the particles’ velocity can be derived, and from that velocity the hydrodynamic diameter of the particles can be calculated by using the Stokes–Einstein equation.

An important factor for making good DLS measurements is using the right concentration. The concentration of particles must be high enough to produce a good scattering intensity. On the other hand, if the concentration is too high, then multiple scattering may occur, which can produce an erroneous result.

Vitamin B1, or thiamine, is an essential mineral that helps the body access energy by providing elements needed for processing carbohydrates. It also helps maintain the central nervous system’s operations. A deficiency in vitamin B1 may result in weight loss, weakness, irregular heart rate and emotional disturbances.

Due to its small size and low molecular weight, vitamin B1 is an ideal sample for identifying the concentration limits of DLS.

In an application report from Anton Paar, an experiment is carried out to show how to determine the most suitable concentration for a DLS measurement of a vitamin B1 sample.

Click on the link above to download the Application Report and to learn about the results of this experiment.

Anton Paar Ltd

Anton Paar develops, produces and distributes highly accurate laboratory instruments and process measuring systems, and provides custom-tailored automation and robotic solutions.

Anton Paar develops, produces and distributes highly accurate laboratory instruments and process measuring systems, and provides custom-tailored automation and robotic solutions.

It is the world leader in the measurement of density, concentration and CO2 and in the field of Rheometry. Other areas of specialty are: Viscometry, Polarimetry, Refractometry, Microwave Sample Preparation & Synthesis, Chemical and Mechanical Surface and Nanostructure Analysis.

With over 2200 employees at the headquarters in Graz (Austria) and the 25 sales subsidiaries worldwide, Anton Paar ensure that their products live up to their excellent reputation. The core competence of the company – high-precision production – and close contact to the scientific community form the basis for the quality of Anton Paar’s instruments.

A strong sales network in more than 110 countries guarantees customers rapid support and answers to their application and service queries.

Innovation is the basis for progress and every year Anton Paar invests more than 20 percent of the annual turnover in R&D.

Anton Paar Limited, a subsidiary of Anton Paar GmbH, provides sales, application support and service to users across the UK and Ireland.

With an office in St Albans (UK) and Dublin (Ireland) and a team of over 30 dedicated employees, Anton Paar Limited aims to provide first-class service at every stage in the customer relationship, ensuring our products live up to their excellent reputation.

Anton Paar Ltd
Unit F, The Courtyard
St Albans
AL4 0LA
UK

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