The engineered living material is a 3D-printed structure made of a seaweed-based polymer combined with bacteria that have been genetically engineered to produce an enzyme that transforms various organic pollutants into benign molecules. The bacteria were also engineered to self-destruct in the presence of theophylline, offering a way to eliminate them once their job was done.
The researchers describe the new decontaminating material in Nature Communications.
“What’s innovative is the pairing of a polymer material with a biological system to create a living material that can function and respond to stimuli in ways that regular synthetic materials cannot,” said Jon Pokorski, a professor of nanoengineering at UC San Diego who co-led the research.
The work was a collaboration among engineers, materials scientists and biologists at the UC San Diego Materials Research Science and Engineering Center (MRSEC). Co-principal investigators of the multidisciplinary team include molecular biology professors Susan Golden and James Golden and nanoengineering professor Shaochen Chen.
“This collaboration allowed us to apply our knowledge of the genetics and physiology of cyanobacteria to create a living material,” said Susan Golden, a faculty member in the School of Biological Sciences. “Now we can think creatively about engineering novel functions into cyanobacteria to make more useful products.”
To create the living material, the researchers used hydrated alginate to make a gel, then mixed it with cyanobacteria before feeding the mixture into a 3D printer.
After testing various 3D-printed geometries for their material, the researchers found that a grid-like structure was optimal for keeping the bacteria alive. The chosen shape has a high surface area to volume ratio, which places most of the cyanobacteria near the material’s surface to access nutrients, gases and light.
According to UCSD, the increased surface area also makes the material more effective at decontamination.
As a proof-of-concept experiment, the researchers genetically engineered the cyanobacteria in their material to continually produce laccase, a decontaminating enzyme.
Laccase can be used to neutralise organic pollutants including bisphenol A (BPA), antibiotics, pharmaceutical drugs and dyes. In this study, the researchers demonstrated that their material can be used to decontaminate the dye-based pollutant indigo carmine, a blue dye widely used in the textile industry. In tests, the material decolourised a water solution containing the dye.
The researchers also developed a way to eliminate the cyanobacteria after the pollutants have been cleared. They genetically engineered the bacteria to respond to theophylline, which triggers the bacteria to produce a protein that destroys their cells.
“The living material can act on the pollutant of interest, then a small molecule can be added afterwards to kill the bacteria,” Pokorski said in a statement. “This way, we can alleviate any concerns about having genetically modified bacteria lingering in the environment.”
The researchers noted that a preferable solution is to have the bacteria destroy themselves without the addition of chemicals and this will be one of the future directions of this research.
“Our goal is to make materials that respond to stimuli that are already present in the environment,” said Pokorski.
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