In a study published in PLOS ONE, Cornell University biomedical engineers and Weill Cornell Medical College physicians described how 3D printing and injectable gels made of living cells can fashion ears that are practically identical to a human ear. Over a three-month period, these flexible ears grew cartilage to replace the collagen that was used to mould them.
‘This is such a win-win for both medicine and basic science, demonstrating what we can achieve when we work together,’ said co-lead author Lawrence Bonassar, associate professor of biomedical engineering.
The novel ear may be the solution reconstructive surgeons have long wished for to help children born with ear deformity, said co-lead author Dr. Jason Spector, director of the Laboratory for Bioregenerative Medicine and Surgery and associate professor of plastic surgery at Weill Cornell in New York City.
‘A bioengineered ear replacement like this would also help individuals who have lost part or all of their external ear in an accident or from cancer,’ Spector said in a statement.
Replacement ears are usually constructed with materials that have a Styrofoam-like consistency, or sometimes, surgeons build ears from a patient’s harvested rib. This option is challenging and painful for children, and the ears rarely look completely natural or perform well, Spector said.
To make the ears, Bonassar and colleagues started with a digitised 3D image of a human subject’s ear, and converted the image into a digitized ‘solid’ ear using a 3D printer to assemble a mould.
This Cornell-developed, high-density gel is similar to the consistency of jam when the mould is removed. The collagen served as a scaffold from which cartilage could grow.
Bonassar said, ‘It takes half a day to design the mould, a day or so to print it, 30 minutes to inject the gel, and we can remove the ear 15 minutes later. We trim the ear and then let it culture for several days in nourishing cell culture media before it is implanted.’
The incidence of microtia, which is when the external ear is not fully developed, varies from almost one to more than four per 10,000 births each year. Many children born with microtia have an intact inner ear, but experience hearing loss due to the missing external structure.
Spector said that the best time to implant a bioengineered ear on a child would be when they are about five or six years old. At that age, ears are 80 percent of their adult size.
If all future safety and efficacy tests work out, it might be possible to try the first human implant of a Cornell bioengineered ear in as little as three years, Spector said.