Microstructured surface reveals amphiphiles to naked eye

The research from the Harvard John A. Paulson School of Engineering and Applied Sciences (SEAS) was published recently in the Proceedings of the National Academy of Science (PNAS).

According to SEAS, the gold standard for testing for endotoxins, a common type of amphiphiles that can contaminate water and can cause severe illness and death, requires the use of compounds only found in the blood of horseshoe crabs, which makes the process expensive and unsustainable. Cheaper alternatives exist but are not sensitive enough to detect amphiphiles at meaningful levels.

The new test, developed by Joanna Aizenberg, the Amy Smith Berylson Professor of Materials Science and Professor of Chemistry & Chemical Biology at SEAS, and Xiaoguang Wang, a former postdoctoral Fellow in Aizenberg lab and now an Assistant Professor of Chemical and Biomolecular Engineering at Ohio State University, uses rolling droplets on microstructured surfaces to detect amphiphiles at ultralow concentrations.

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The research team demonstrated the technique to detect levels of pathogenic amphiphilic endotoxins in water, compounds that can be toxic even at extremely low concentrations.

The microstructured surface is said to be made up of thousands of circular micropillars, evenly coated with self-assembled long-tailed molecules, creating a smooth, friction-free interface except for at the edges of the micropillars where there are gaps in the coating. When a droplet with no amphiphile molecules rolls down the surface it will hit a gap and stop, unable to overcome the friction of the disordered edge.

A droplet with higher levels of amphiphiles will continue to roll because amphiphile molecules will co-assemble with long-tailed molecules and fill the gaps in the surface. In the case of endotoxins, as amphiphile molecules are deposited onto the surface, they connect with other amphiphile molecules in the droplet, building larger and larger compounds which eventually slow and stop the droplet. Where the droplet stops on the surface tells scientists how many gaps it has patched, revealing the concentration of amphiphiles.

“Our surfaces provide a rapid and portable method of detecting amphiphiles within droplets that you can see with your bare eyes,” Aizenberg said in a statement. “No other methods allow detection at the low levels that we’re seeing in our tests, without using expensive or sophisticated equipment.”

The researchers also developed a model to predict how different amphiphile compounds at different concentrations would interact with the structured surface. By changing the size, shape and distance between pillars, as well as the molecular coating, the surface can be tuned to detect specific types of amphiphiles at specific concentrations.

“Our method is broadly applicable to any kind of amphiphile,” said Wang. “With this general method, we are already detecting endotoxins at levels that are relevant for water quality testing, but the test can also be further optimised to detect even lower concentrations.”