Nanomeshes are being fabricated in a way that makes them effective at delivering antibiotics, a breakthrough that could help in the global fight against antibiotic resistance.
The breakthrough has been made by researchers at Flinders University in Australia and collaborators in Japan. In studying the effectiveness of the nanomesh, two antibiotics, Colistin and Vancomycin, were added together with gold nanoparticles to the mesh, before they were tested over a 14-day period.
Flinders Institute for Nanoscience and Technology Associate Professor Ingo Koeper said 20cm by 15cm pieces of mesh were produced which contain fibres 200nm in diameter.
“In order to deliver the antibiotics to a specific area, the antibiotics were embedded into the mesh produced using a technique called electrospinning, which has gained considerable interest in the biomedical community as it offers promise in many applications including wound management, drug delivery and antibiotic coatings,” Koeper said.
“A high voltage is then applied between the needle connected to the syringe, and the collector plate which causes the polymer solution to form a cone as it leaves the syringe, at which point the electrostatic forces release a jet of liquid.”
“Small charged nanoparticles altered the release of the antibiotics from the nanomesh. The addition of gold nanoparticles likely neutralised charge, causing the antibiotic to migrate toward the centre of the fibre, prolonging its release.”
According to Flinders, the results also suggest dosages could be reduced compared to traditional drugs which can also diminish potential side effects and complications.
“Although the dosage is reduced compared to an oral dosage, the concentration of antibiotics delivered to the infection site can still be higher, ensuring the bacteria cannot survive which will reduce instances of resistance.”
“This research, as a proof of concept, suggests an opportunity for fabricating nanomeshes which contain gold nanoparticles as a drug treatment for antibiotics.”
Working with Dr Harriet Whiley, a Flinders environmental health scientists, the researchers studied how the release of the drugs affected the growth of E. Coli. The in vitro study confirmed Colistin with negatively charged gold nanoparticles produced the most efficient nanomesh, significantly affecting bacterial growth.
“Further investigation is needed to determine if other small charged particles affect the release of drugs and how it affects the release over time. As it is a pharmaceutical application, the stability of the mesh under different storage conditions as well as the toxicological properties also need to be evaluated.”
Findings from the research have been published in RSC Advances.